| Group | Club/Team Name | Team ID | Head Coach |
| East 1 | Nashville United Soccer Academy - 01 NUSA B12 Maroon |
0534-01AB2U-0047 | Calvin da Cunha |
| West 1 | Lobos Rush (Collierville Soccer Association) 2012 Lobos Rush Blue |
0603-01SB2U-2346 | Richard Keene |
| East 2 | FC Alliance Knoxville FC Alliance 2012B Pre-ECNL |
0204-021B3U-0223 | Caleb Lucas |
| West 2 | Midsouth Futbol Midsouth Bartlett 2012 Boys Navy |
0654-01SB2U-0096 | Jason Love |