| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Clarksville Soccer Club Clarksville SC - B2010 Maroon |
0422-012B4U-0011 | Alejandro Herrera |
| A2 | Chattanooga FC Academy Chattanooga FC - 2010B Navy |
0316-022B4U-7928 | Derek Greene |
| A3 | TN Tempo FC Academy TN Tempo FC Academy - 2010B |
0592-012B4U-0012 | Nathaniel Bedai |
| A4 | Lobos Rush (Collierville Soccer Association) 2010 Lobos Rush E64 |
0603-012B4U-0035 | Zayrion Harris |
| A5 | AYSO Alliance Knoxville - 01 AYSOR124 ALLIANCE 10B |
0292-013B4U-0026 | Clement Hendrickson |
| A6 | Action Elite Soccer Club Action Elite 2010B |
0501-012B4U-0001 | Zachary Cannon |
| A7 | Wilson United Soccer Club Wilson United SC 2010 B Black |
0406-012B4U-0003 | Joel Gibbs |
| A8 | Music City Soccer Club Music City FC 10B Premier |
0517-013B4U-3500 | Francisco Aleman |