| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2010 Lady Lobos Rush E64 |
0603-011G4U-0021 | Collin Scott |
| A2 | Chattanooga FC Academy Chattanooga FC - 2010G Navy |
0316-02GG4U-7947 | Juan Hernandez Mendizabal |
| A3 | Tennessee Soccer Club TSC 2011 Girls ECNL |
0522-011G3U-0051 | Dalton Kaufman |
| A4 | Tennessee United Soccer Club TUSC 2010 Girls DPL |
0424-011G4U-0002 | Eduardo Carvacho |
| A5 | One Knoxville One Knox 10G- DPL |
0293-01D1G4U-0014 | Kelvin Scott |
| A6 | Germantown Legends Germantown Lady Legends 2010 Black |
0606-011G4U-0002 | Lance Loeffel |
| A7 | Tennessee Soccer Club TSC 2010 Girls Nike |
0522-011G4U-0052 | Lance Mayes |
| A8 | FC Alliance Knoxville FC Alliance 2011G ECNL |
0204-02AG4U-0242 | Elaine Roth |