| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Germantown Legends Germantown Lady Legends 09 |
0606-012G5U-0017 | Ricky Allen |
| A2 | All-In FC TN All-In FC TN 09/10G Phantom |
0443-01SLG5U-0002 | Davis Draper |
| A3 | Soccer Ole OLE FC 09G Premier |
0632-021G5U-0023 | Evan Hacker |
| A4 | Camp Forest Football Club CFFC 2009/2010 Girls |
0523-022G5U-0012 | Jacob Cashion |
| A5 | Nashville United Soccer Academy - 01 NUSA G09 Navy |
0534-012G5U-0016 | Stephanie Wallace |
| A6 | Tennessee Soccer Club TSC Sumner 2009 Girls Showcase |
0522-012G5U-2208 | Zack Trobaugh |
| A7 | One Knoxville One Knox 09G- Orange |
0293-01D2G5U-0015 | Christopher Shumate |
| A8 | Midsouth Futbol Midsouth 2009 Girls |
0654-012G5U-0109 | David Burns |