| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee United Soccer Club TUSC 2009 Girls DPL |
0424-011G5U-0003 | Nduka Kemjika |
| A2 | Tennessee Soccer Club TSC 2009 Girls Nike |
0522-011G5U-0054 | Robert Taylor |
| A3 | Kononia Soccer Club - 01 Kononia Lady Fire 2009/10s |
0611-011G5U-1985 | Sean Cogan |
| A4 | Tennessee Soccer Club TSC 2010 Girls ECNL |
0522-011G4U-2217 | Dean Blain |
| A5 | One Knoxville One Knox 09G- DPL |
0293-01D1G5U-0003 | Derrick Long |
| A6 | Nashville United Soccer Academy - 01 NUSA G09 Maroon |
0534-011G5U-0003 | Adam Harris |
| A7 | Lobos Rush (Collierville Soccer Association) 2009 Lady Lobos Rush E64 |
0603-011G5U-0019 | Traci Sipniewski |
| A8 | Kings Hammer KHM 09G Premier I |
0515-01AG5U-0215 | Alan Oates |