| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy - 2008 Girls |
0324-021G6U-0056 | Daniel McBride |
| A2 | Germantown Legends Germantown Lady Legends 08 Black |
0606-011G6U-0003 | Carey Talley |
| A3 | Kings Hammer KHM 08G Premier I |
0515-01AG6U-0214 | Alan Oates |
| A4 | Tennessee United Soccer Club TUSC 2008 Girls DPL |
0424-011G6U-0004 | Nathan Harrell |
| A5 | One Knoxville One Knox 08G- DPL |
0293-01D1G6U-0004 | Brenna Lacaillade |
| A6 | Clarksville Soccer Club Clarksville SC - G2008 Maroon |
0422-011G6U-0009 | David Hite |
| A7 | International Futbol Association IFA-Lady Futsal Escola 2008 |
0650-501G6U-0334 | Lawrence Kuhlman |
| Bye | Nashville United Soccer Academy - 01 NUSA G08 Maroon |
0534-011G6U-0004 | Racheal Foxley |