| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2007 Lady Lobos Rush Elite |
0603-012G7U-2359 | Jenna Odum |
| A2 | Tennessee Soccer Club TSC Williamson 2007 Girls Showcase |
0522-012G7U-2303 | Sarah Richardson |
| A3 | Jackson Soccer Club JSC 2007 Girls |
0609-01DG7U-9919 | Armando Guell Castro |
| A4 | Nashville United Soccer Academy - 01 NUSA G07 Maroon |
0534-011G7U-0005 | Ethan Keogh |
| A5 | Kings Hammer KHM 07G Premier I |
0515-01BG7U-0213 | Jason Morrison |
| A6 | One Knoxville One Knox 07G- Orange |
0293-01D2G7U-0018 | Joshua Martin |
| A7 | Stones River Futbol Club Stones River FC 2007 Girls |
0521-012G7U-0024 | Charles Bozman |
| A8 | Tennessee Soccer Club TSC Williamson 2007 Girls Premier |
0522-012G7U-2304 | Matheus Mello |