| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tri-Cities United Soccer Club Tri-Cities United 06/07 Girls Premier |
0106-011G8U-0003 | Filipe Gomes Barateiro |
| A2 | Germantown Legends Germantown Lady Legends 06 |
0606-012G8U-0045 | Scott Melton |
| A3 | Lincoln Futbol Club Lincoln Futbol Club - G18U |
0514-012G8U-0050 | Thomas Franklin |
| A4 | Camp Forest Football Club CFFC 2006 Girls |
0523-022G8U-0011 | Katie Reid |
| A5 | One Knoxville One Knox 06/07G- Orange |
0293-01D2G8U-0019 | Mark Williams |
| A6 | Wilson United Soccer Club Wilson United SC 2006 G Black |
0406-012G8U-0025 | James Branch |