| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 05/06 Lady Lobos Rush E64 |
0603-011G9U-2353 | Mark Forrest |
| A2 | Nashville United Soccer Academy - 01 NUSA G06 Maroon |
0534-011G9U-0006 | n/a |
| A3 | One Knoxville One Knox 06/05G- DPL |
0293-01D1G9U-0006 | Derrick Long |